Abstract / Excerpt:

This study aimed to determine the effectiveness of omega-3 fatty acids against experimentally induced colitis in mice. Four groups with five mice each were used in the experiment, with three groups being induced with colitis through a single intra-colonic application of 0.1 milliliters of 5% trinitrobenzene sulfonic acid into the descending colon. One group was administered a single dosage of omega-3 fatty acids while another administered a double dosage. Both groups were treated for a period of two weeks, with the weight and stool appearance of each mouse being carefully monitored. After which, all twenty mice were euthanized and dissected for colon extraction. Slide preparations of the tissue samples were made by the proponents with the assistance of the pathologist of the diagnostic center. The data was obtained through a histopathological examination of the prepared slides by a pathologist who created a scoring system to determine the degree of inflammation of each subject. The scores were then subjected to statistical analysis. The results show a two-week normal dosage treatment of omega-3 fatty acids and a two-week double dosage treatment of omega-3 fatty acids both do not present histopathological changes in the descending colon of mice induced with ulcerative colitis. The study revealed that a treatment of omega-3 fatty acids is not effective in reducing the level of inflammation in mice induced with ulcerative colitis.

Info

Source Institution	Ateneo de Davao University
Unit	Natural Science and Mathematics
Authors	Alicer, John Felix P., Billanes, Francis Louise B., Caballes, Nadine V.
Page Count	59
Place of Publication	Davao City
Original Publication Date	March 1, 2014
Tags	Essential fatty acids in human nutrition, Health Aspects, Omega-3 Fatty Acids

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